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  • Indentifying Species with DNA Barcoding « Barcode of Life
    DNA barcoding and all related activities You will find up to date information about CBOL on this website under About What is CBOL If you would like to look for material on the old CBOL website please click here If

    Original URL path: http://barcoding.si.edu/ (2015-06-03)
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  • Consortium for the Barcode of Life
    Life CBOL is an international initiative devoted to developing DNA barcoding as a global standard for the identification of biological species DNA barcoding is a new technique that uses a short DNA sequence from a standardized and agreed upon position in the genome as a molecular diagnostic for species level identification DNA barcode sequences are very short relative to the entire genome and they can be obtained reasonably quickly and cheaply The Folmer region at the 5 end of the cytochrome c oxidase subunit 1 mitochondrial region COI is emerging as the standard barcode region for almost all groups of higher animals This region is 648 nucleotide base pairs long in most groups and is flanked by regions of conserved sequences making it relatively easy to isolate and analyze A growing number of studies have shown that COI sequence variability is very low generally less than 1 2 and that the COI sequences of even closely related species differ by several percent making it possible to identify species with high confidence For those groups in which COI is unable to resolve species level differences CBOL recommends the use of an additional gene region In some groups COI is not an effective barcode region and a different standard region must be identified In all cases DNA barcoding is based on the use of a short standard region that enables cost effective species identification To learn more about DNA barcoding see Barcode of Life Initiative PDF 1 83Mb DNA Barcoding A New Tool for Identifying Biological Specimens and Managing Species Diversity English PDF 858Kb Farsi PDF 3Mb Barcoding Life Ten Reasons PDF 437Kb and The Barcode Blog CBOL has more than 170 Member Organizations from more than 50 countries including Natural history museums zoos herbaria and botanical gardens University departments of biology

    Original URL path: http://barcoding.si.edu/Originalindex.html (2015-06-03)
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  • Consortium for the Barcode of Life
    are distinct Until now biological specimens were identified using morphological features In some cases a trained technician could make routine identifications using morphological keys but in most cases an experienced professional taxonomist is needed If a specimen is damaged or is in an immature stage of development even specialists may be unable to make identifications Barcoding solves these problems because non specialists can obtain barcodes from tiny amounts of tissue This is not to say that traditional taxonomy has become less important but rather that DNA barcoding can serve a dual purpose as a new tool in the taxonomists toolbox supplementing his her knowledge as well as being an innovative device for non experts who need to make a quick identification The gene region that is being used as the standard barcode for almost all animal groups is a 648 base pair region in the mitochondrial cytochrome c oxidase 1 gene CO1 COI is proving highly effective in identifying birds butterflies fish flies and many other animal groups COI is not an effective barcode region in plants because it evolves too slowly but botanists are now close to identifying a combination of gene regions that will serve as a barcode region for plants Barcoding projects have four components The Specimens Natural history museums herbaria zoos aquaria frozen tissue collections seed banks type culture collections and other repositories of biological materials are treasure troves of identified specimens The Laboratory Analysis Barcoding protocols pdf 561Kb can be followed to obtain DNA barcode sequences from these specimens The best equipped molecular biology labs can produce a DNA barcode sequence in a few hours for as little as 5 per specimen The data are then placed in a database for subsequent analysis The Database One of the most important components of the Barcode Initiative

    Original URL path: http://barcoding.si.edu/whatis.html (2015-06-03)
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  • Consortium for the Barcode of Life
    bird airplane collisions and avian blood samples from biting insects that harbor West Nile virus or other human disease agents Bee BOL the Bee Barcode of Life Initiative is a global effort to coordinate the assembly of a standardized reference sequence library for all 20 000 bee species Bee BOL is creating a valuable public resource in the form of an electronic database containing DNA barcodes images and geospatial coordinates of examined specimens The database contains linkages to voucher specimens information on species distributions nomenclature authoritative taxonomic information collateral natural history information and literature citations ECBOL is an information and coordination hub on DNA barcoding in Europe organized within EDIT the European Institute of Taxonomy and maintained by CBS the Centraalbureau voor Schimmelcultures in Utrecth The ECBOL initiative Calibrating European Biodiversity using DNA Barcodes is a network of European researchers and is seeking to obtain funding fro the coordination and maintenance of a Network of European Leading Labs FISH BOL the Fish Barcode of Life campaign is collecting barcodes from at least five specimens representing the 30 000 species of marine freshwater and estuarine fish of the world Like ABBI FISH BOL has a central Steering Committee and Regional Working Groups MarBOL is an international campaign to obtain at least 50 000 barcode records of marine species by October 2010 MarBOL is led by an international Steering Committee and an affiliated project of the Census of Marine Life CoML MBI the Mosquito Barcode Initiative is another demonstration project aimed at producing a global operational system for identifying mosquitoes in two years MBI plans to barcode at least five specimens from 80 of the 3200 known mosquito species Disease bearing species and their closest relatives will be the highest priority TBI the Tephritid Barcode Initiative is a two year demonstration project

    Original URL path: http://barcoding.si.edu/major_projects.html (2015-06-03)
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  • Consortium for the Barcode of Life
    Links Vision CBOL and BOLI share the same basic vision Developing DNA barcoding as a global standard for species identification CBOL s Mission CBOL works with its international partners to catalyze The creation of a public database of barcode reference sequences linked to voucher specimens and associated biological information The formation of barcoding projects that will contribute an accelerating flow of barcode records into the public database The solution of technical logistical and structural problems encountered by researchers and users in the Barcode of Life Initiative The development of new technologies that will make DNA barcoding faster more accurate more portable and less expensive The involvement of researchers and users of barcode data from all regions of the world especially those with high biodiversity The development of intellectual activity involving DNA barcode data in the wider academic community among diverse users throughout society Greater awareness of DNA barcoding beyond taxonomic researchers and The use of barcode data for the benefit of science and society CBOL s Strategy The CBOL Secretariat will pursue its mission by identifying and catalyzing international partnerships among the following stakeholders in barcoding The Taxonomic Community Natural history museums herbaria zoos aquaria and universities and the taxonomists that work therein The User Community Governmental and intergovernmental agencies conservation organizations and other NGOs private sector companies and other organizations that use taxonomic information in pursuit of their applied missions The Service Community Research organizations and private sector companies in the areas of genetics bioinformatics and biotechnology that can provide services to barcoding projects and the Barcode Initiative and The Funding Community Public and private foundations governmental and intergovernmental agencies and other organizations that support basic and applied research The Access and Benefits Sharing Brochure is a document that can be used by scientists to explain the process of

    Original URL path: http://barcoding.si.edu/vision.html (2015-06-03)
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  • Consortium for the Barcode of Life
    able to distinguish species and identify specimens including incomplete damaged or immature specimens using a very short gene sequence immediately captured the imagination of many taxonomists geneticists and evolutionary biologists The first of two exploratory workshops both sponsored by the Alfred P Sloan Foundation was held at the Cold Spring Harbor Banbury Conference Center on March 9 12 2003 The workshop was entitled DNA and Taxonomy and the article it produced pdf 159Kb stressed the potential applications of such a technique for taxonomy and the potential benefits to society It argued that if DNA barcoding could be proven feasible across a wide range of taxonomic groups then rapid inexpensive assignment of unidentified specimens to known species could be done by non specialists This would expand the impact of taxonomic research beyond the confines of academic circles and would provide benefits to agriculture public health education and many other segments of society A second workshop Taxonomy DNA and the Barcode of Life was held at the Banbury Center on September 10 12 2003 As reflected in the Banbury 2 conference report pdf 94Kb the discussion was much more focused than at the previous Banbury I workshop Cytochrome c oxidase subunit I was emerging as a strong candidate to be the DNA barcode region for eukaryotic animals Participants developed a blueprint for an international Bacode of Life Project that evolved quickly into a grant proposal to the Sloan Foundation In April 2004 the Sloan Foundation made a 30 month 669 000 award to the Smithsonian Institution for the creation and support of a Consortium for the Barcode of Life Dr Scott Miller of the Smithsonian Institution is the Principal Investigator on the grant from the Sloan Foundation CBOL held its inaugural meeting at the Smithsonian Institution s National Museum of Natural History

    Original URL path: http://barcoding.si.edu/history.html (2015-06-03)
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  • Consortium for the Barcode of Life
    Search Sitemap text size Get Involved in CBOL Find out how you can get involved Learn about major CBOL projects and other barcode initiatives Browse Case Studies of barcoding projects Propose a barcoding project post a Case Study and find partners Submit barcode data Examine barcode data Join CBOL Sponsor Links Governance CBOL consists of Member Organizations that share an interest in DNA barcoding and work together to promote its development CBOL pursues this mission through a variety of activities that are overseen by the CBOL Executive Committee and are managed by a Secretariat Office CBOL s Executive Committee has adopted Terms of Reference pdf 45Kb under which the Consortium operates Member Organizations Since its inaugural meeting in May 2004 over 150 organizations from 45 countries across 6 continents have joined the Consortium for the Barcode of Life View CBOL members on the world map The Executive Committee oversees the organization and reports to the Member Organizations An Implementation Board IB consists of the Working Group chairs and leaders of all major CBOL activities and liasons to important partner activities such as GenBank and the Canadian Barcode Network Four Working Groups develop particular aspects of DNA Barcoding The Secretariat Office

    Original URL path: http://barcoding.si.edu/governance.html (2015-06-03)
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  • Consortium for the Barcode of Life
    Barcode of Life Data Systems BOLD Canadian Barcode of Life Network BOLNET Canadian Centre for DNA Barcoding Global Biodiversity Information Facility GBIF Catalogue of Life Search Sitemap text size Get Involved in CBOL Find out how you can get involved Learn about major CBOL projects and other barcode initiatives Browse Case Studies of barcoding projects Propose a barcoding project post a Case Study and find partners Submit barcode data Examine

    Original URL path: http://barcoding.si.edu/new_and_updates.html (2015-06-03)
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