archive-edu.com » EDU » V » VANDERBILT.EDU

Total: 1851

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Mass Spectrometry | VIIBRE | Vanderbilt University
    Technical Staff Faculty Fellows External Associates Scientific Steering Committee Scientific Advisory Board Industrial Advisory Board Accomplishments In the News Publications Patents Presentations Portal Links Home Mass Spectrometry Mass Spectrometry The VIIBRE Automated Biosystems Core s Omni Omics system is built around a Waters Synapt G2 Ion Mobility Mass Spectrometer IM MS an automated Nikon Eclipse microscope and other real time instruments and microfabricated bioreactors for sensing and controlling small populations

    Original URL path: http://www.vanderbilt.edu/viibre/massspectrometry.php (2016-02-16)
    Open archived version from archive

  • Organs-on-a-Chip and Microphysiological Systems | VIIBRE | Vanderbilt University
    and learn about the communication and control of biological systems at the scale of individual organs on chips Allometric scaling provides some guidance but appropriate biochemical and functional scaling of multiple organs and a universal cell culture medium are critical to proper systems function and valid pharmacological interpretation The ultimate potential of engineered organs or microphysiological systems will be realized by more complex powerful and integrated systems capable of recapitulating inter and intra organ signaling and dynamics The operation of a single HoC requires a perfusion system functionally integrated into a tubing circuit with connections for medium inflow and outflow pressure measurement sample removal pumps to recirculate and replenish culture medium and sensors actuators and control electronics to regulate flow rates pH temperature and gas mixtures Real time measurements of glucose lactate pH and oxygen allow the quantification of HoC metabolism including aerobic vs anaerobic processes receptor activation and other activities that affect metabolism Concept of Planar BBB NVU Chip The Vanderbilt Institute for Integrative Biosystems Research and Education VIIBRE is currently building a planar Neurovascular Unit NVU on a Chip under NIH funding VIIBRE is also building a cardiac papillary muscle on a chip utilizing DTRA funding and additional supplementary funding was received to research a Retinal Blood Barrier on a Chip In addition VIIBRE is developing a hardware and multi organ chip integration platform for the DTRA X C E L program in collaboration with organ on chip teams from Charité Universitätsmedizin Berlin liver Harvard University heart Los Alamos National Laboratory lung organ integration and integrated validation University of California San Francisco kidney VIIBRE OoC Publications Lab on a Chip Review Article Scaling and systems biology for integrating multiple organs on a chip Wikswo JP Curtis EL Eagleton ZE Evans BC Kole A Hofmeister LH Matloff WJ Scaling

    Original URL path: http://www.vanderbilt.edu/viibre/organs-on-a-chip.php (2016-02-16)
    Open archived version from archive

  • Structural Mass Spectrometry | VIIBRE | Vanderbilt University
    the drift tube can then be converted into a collision cross section value which is representative of a rotationally averaged surface area A schematics of an ion mobility drift tube to show ions separating based on their size The sample data shows that a shorter drift time corresponds to a smaller conformation while a longer drift time corresponds to a larger conformation Collision cross section values are then derived for these conformations providing a rotationally averaged surface area for the molecule A correlation can then be made between the mass to charge ratios and the collision cross section values resulting in a conformational landscape Previous studies show that different classes of biomolecules separate out in this conformational landscape oligonucleotides carbohydrates peptides lipids allowing for the separation and identification of these classes in complex biological samples which are common in systems biology 1 These biological classes separate in conformational space due to their unique and differentiating structural characteristics The data from an IM MS experiment can be plotted as shown above generating a conformational space landscape The mass to charge ratio is plotted against the CCS values A cartoon of the data is shown on the left and actual data is plotted on the right The different classes of biomolecules separate out in conformational space due to similar structural characteristics within their classes Structural IM MS studies are often supplemented with computational work that samples the conformational space of the molecules of interests in silico These computational studies consists of two steps 1 Computationally sampling the conformational space of the molecule with molecular dynamics or other sampling methods followed by 2 A theoretical determination of the CCS of each of these generated conformations Experimental CCS values can then be used to discriminate the theoretically generated conformations The resulting conformations can then

    Original URL path: http://www.vanderbilt.edu/viibre/structural-mass-spec.php (2016-02-16)
    Open archived version from archive

  • VIIBRE - The Teaching of Innovation | VIIBRE | Vanderbilt University
    and medicine at all levels SyB BURE students become adept at a wide assortment of skills important for systems biology including methods of making BioMEMS devices computational modeling of flow diffusion and heat transfer with MATLAB and COMSOL the basics of cell culture microscopy and image processing and most important effective communication of their results to the broader scientific community Much of the technical training has been incorporated at the request of the students who realize as they approach their research projects that certain quantitative tools are needed so while some aspects of the training are dictated by the mentors the students have an active say in what things are important to learn and know The students are also active participants in scientific meetings including BMES EMBS microTAS and q bio and have helped to organize and run workshops at many meetings To date twelve papers published in peer reviewed journals and four patents and patent applications include SyBBURE students as co authors and co inventors In its broadest sense SyBBURE gives students a larger view of the needs and consequences of interdisciplinary science and the thrill of innovation The program involves the students in the immediacy and relevance of systems biology and bioengineering highly quantitative analytical fields that together are enabled by tools techniques and theories from across the biological medical mathematical engineering and physical science disciplines and it gives them the experience of working in teams and mentoring each other of formulating problem driven questions and of developing new technology New Undergraduate and Graduate Courses Since its inception VIIBRE has worked to improve the undergraduate graduate and postdoctoral research experience through classroom and laboratory training and research In support of these efforts and in his role as the Gordon A Cain University Professor John Wikswo has offered two

    Original URL path: http://www.vanderbilt.edu/viibre/teachinginnovation.php (2016-02-16)
    Open archived version from archive

  • SyBBURE | VIIBRE | Vanderbilt University
    Scientific Steering Committee Scientific Advisory Board Industrial Advisory Board Accomplishments In the News Publications Patents Presentations Portal Links Home SyBBURE SyBBURE A generous gift from Gideon Searle a Vanderbilt alumnus gives undergraduate students a distinctive opportunity to explore science at one of its most promising and exciting frontiers the intersection of systems biology and bioengineering The aim of the Searle gift was to provide undergraduate students with mentored experiences in advanced scientific investigation with some of the University s leading research and teaching faculty SyBBURE Systems Biology and Bioengineering Undergraduate Research Experience is directed by Assistant Professor of the Practice of Biomedical Engineering Kevin Seale who also leads a group of students who are using microfabricated nanophysiometers to study various cell populations including cells of the immune system and Saccharomyces cerevisiae It s great preparation for graduate school where you spend several years working toward one goal Brittany Rohrman BA 2009 SyBBURE gave me a broad deep exposure to several research areas That s uncommon for undergraduate research Arunan Skandarajah BE MS 2009 Members of SyBBURE come from different science and engineering fields so everyone brings unique insights to discussions I was exposed to ideas that I otherwise would not

    Original URL path: http://www.vanderbilt.edu/viibre/sybbure.php (2016-02-16)
    Open archived version from archive

  • SyBBURE - Education | VIIBRE | Vanderbilt University
    Presentations Portal Links Home SyBBURE Education SyBBURE Education Many top U S universities recognize that a greater focus on experiential learning may lead to more engaged and better trained science math and engineering students VIIBRE faculty set early trends in this area by initiating a privately funded undergraduate research program the Systems Biology and Bioengineering Undergraduate Research Experience SyBBURE Our guiding educational principle is that human beings are natural born experimentalists who learn best by direct first person observation and subsequent experimentation After all this is how children learn to crawl walk talk and do most everything in their first years Education through the college level necessarily abstracts and removes the student from the first person role by presenting condensed information in a lecture or textbook We seek to reinvigorate the learning process by taking students to the very boundary of the unknown where the answers to important questions must be determined by observation and strategic experimentation Our educational philosophy is interdisciplinary by definition Scientific investigation requires whatever knowledge the problem dictates which means that biology students may need and want to learn about computer programming to improve their experiments and likewise a physics or engineering student may quickly learn

    Original URL path: http://www.vanderbilt.edu/viibre/education.php (2016-02-16)
    Open archived version from archive

  • SyBBURE - Professional Development | VIIBRE | Vanderbilt University
    Publications Research Interests Teaching CV David Cliffel Shane Hutson John A McLean Christina C Marasco CV Administrative Staff Technical Staff Faculty Fellows External Associates Scientific Steering Committee Scientific Advisory Board Industrial Advisory Board Accomplishments In the News Publications Patents Presentations Portal Links Home SyBBURE Professional Development SyBBURE Professional Development The Systems Biology and Bioengineering Undergraduate Research Experience SyBBURE was established as a promising solution to a nationally recognized need for more experiential learning outside the classroom Through SyBBURE undergraduate students engage in long term authentic research projects driven by important scientific questions for which no one knows the answer We seek to help students develop professionally in the context of their own projects by requiring research presentations oral presentations poster presentations and written documents Students whose research has reached maturity are encouraged to travel to scientific meetings such as those of the Biomedical Engineering Society q bio and the Institute of Electrical and Electronics Engineers In this way the students learn for themselves how best to dress network and communicate their results and their career ambitions to the greater scientific community We also recognize the important role of publications in professional development and seek appropriate outlets for publishing the research

    Original URL path: http://www.vanderbilt.edu/viibre/professional-development.php (2016-02-16)
    Open archived version from archive

  • Interdisciplinary Graduate Courses | VIIBRE | Vanderbilt University
    Research Interests Teaching CV David Cliffel Shane Hutson John A McLean Christina C Marasco CV Administrative Staff Technical Staff Faculty Fellows External Associates Scientific Steering Committee Scientific Advisory Board Industrial Advisory Board Accomplishments In the News Publications Patents Presentations Portal Links Home Interdisciplinary Graduate Courses Interdisciplinary Graduate Courses Chemistry 211 Instrumental Analysis Spring 2014 John A McLean The content of this course covers contemporary instrumentation and measurement strategies for chemical analysis The five primary areas include i experimental design and data acquisition strategies ii spectroscopic methodologies iii spectrometric methodologies iv separation technologies and v electrochemical techniques There is a particular emphasis on fundamental considerations in the selection design modeling and construction of advanced instrumentation and experimental constructs in life sciences and materials characterization For a description of Professor John Wikswo s Spring 2014 course please follow the link PDF Chemistry 311 Advanced Analytical Chemistry I Fall 2013 John A McLean The content of this course covers three primary areas i experimental design and data acquisition strategies ii quantitative and qualitative mass spectrometry and iii contemporary proteomic techniques These topics are discussed in the context of advanced mass spectrometry with a particular emphasis on fundamental considerations in the selection design modeling

    Original URL path: http://www.vanderbilt.edu/viibre/grad-courses.php (2016-02-16)
    Open archived version from archive